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tta advanced tet  (TaKaRa)


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    Structured Review

    TaKaRa tta advanced tet
    Tta Advanced Tet, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tet+off+vector/pTet-Off+Advanced+Vector/bio_rxiv__2025__08__20__670374-198-15-24
    Average 95 stars, based on 82 article reviews
    tta advanced tet - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Construct:

    Article Title: Cell biological analysis of DT40 knockout cell lines for cell-cycle genes.
    Article Snippet: DT40 is a chicken B cell line that has been widely used as a model for gene functional studies because the high level of homologous recombination in DT40 cells allows targeted disruption of a gene of interest.. While our laboratory uses DT40 cells to understand kinetochore assembly and function, the approach is applicable to functional studies of other genes that are required for cell cycle progression.. Protocols are presented for the creation of knockout cells and subsequent cell biological analyses for characterizing the phenotypes of these cells.

    Generated:

    Article Title: Cell biological analysis of DT40 knockout cell lines for cell-cycle genes.
    Article Snippet: DT40 is a chicken B cell line that has been widely used as a model for gene functional studies because the high level of homologous recombination in DT40 cells allows targeted disruption of a gene of interest.. While our laboratory uses DT40 cells to understand kinetochore assembly and function, the approach is applicable to functional studies of other genes that are required for cell cycle progression.. Protocols are presented for the creation of knockout cells and subsequent cell biological analyses for characterizing the phenotypes of these cells.

    Cloning:

    Article Title: Cell biological analysis of DT40 knockout cell lines for cell-cycle genes.
    Article Snippet: DT40 is a chicken B cell line that has been widely used as a model for gene functional studies because the high level of homologous recombination in DT40 cells allows targeted disruption of a gene of interest.. While our laboratory uses DT40 cells to understand kinetochore assembly and function, the approach is applicable to functional studies of other genes that are required for cell cycle progression.. Protocols are presented for the creation of knockout cells and subsequent cell biological analyses for characterizing the phenotypes of these cells.

    Plasmid Preparation:

    Article Title: Cell biological analysis of DT40 knockout cell lines for cell-cycle genes.
    Article Snippet: DT40 is a chicken B cell line that has been widely used as a model for gene functional studies because the high level of homologous recombination in DT40 cells allows targeted disruption of a gene of interest.. While our laboratory uses DT40 cells to understand kinetochore assembly and function, the approach is applicable to functional studies of other genes that are required for cell cycle progression.. Protocols are presented for the creation of knockout cells and subsequent cell biological analyses for characterizing the phenotypes of these cells.

    Article Title: The Robo4 cytoplasmic domain is dispensable for vascular permeability and neovascularization
    Article Snippet: .. A mouse Robo4 fragment lacking >95% of the cytoplasmic domain (aa 1–522) was fused with GFP ( ) and inserted 3' to the tetracycline-responsive element (TRE)-CMV promoter of the TET-off vector (Clontech). ..

    Article Title: Thioredoxin-interacting protein regulates the differentiation of murine erythroid precursors.
    Article Snippet: Thioredoxin-interacting protein regulates the differentiation of murine erythroid precursors Jadwiga J. Gasiorek, Marc Mikhael, Daniel Garcia-Santos, Simon T. Hui, Prem Ponka, and Volker Blank Lady Davis Institute for Medical Research, Montreal, Quebec, Canada; Department of Medicine, McGill University, Montreal, Quebec, Canada; Department of Physiology, McGill University, Montreal, Quebec, Canada; Division of Cardiology, Department of Medicine, University of California, Los Angeles, CA, USA

    Article Title: The SYT-SSX Fusion Protein Down-Regulates the Cell Proliferation Regulator COM1 in t(x;18) Synovial Sarcoma
    Article Snippet: .. SYO-1 cells containing Tet-Off vector (Clontech) were isolated with G418 sulfate (1 mg/ml). ..

    Article Title: Calcium channel proteins
    Article Snippet: In the Clontech Tet OFF system the gene is not expressed (or expressed at very low levels) in the presence of doxycycline and expressed at very high levels in the absence of doxycycline. .. The pETE vector is derived from the commercially available Tet OFF vector (Clontech) by inserting an EBNA-1 element to confer an episomal property on the vector. ..

    Article Title: Epidermal Growth Factor Receptor Variant III–Induced Glioma Invasion Is Mediated through Myristoylated Alanine-Rich Protein Kinase C Substrate Overexpression
    Article Snippet: .. EGFRvIII was cloned into the tetracycline-inducible Tet-Off vector (pTRE; Clontech) and cotransfected with the regulatory plasmid (pTA) into human U87 and U373 astrocytoma cells. .. Clones expressing the pTRE-EGFRvIII (U87vIII and U373vIII) or the empty pTRE vector (U87P and U373P) were grown in DMEM supplemented with 10% tetracycline-approved fetal bovine serum (Clontech).

    Isolation:

    Article Title: The SYT-SSX Fusion Protein Down-Regulates the Cell Proliferation Regulator COM1 in t(x;18) Synovial Sarcoma
    Article Snippet: .. SYO-1 cells containing Tet-Off vector (Clontech) were isolated with G418 sulfate (1 mg/ml). ..

    Article Title: The SYT-SSX Fusion Protein Down-Regulates the Cell Proliferation Regulator COM1 in t(x;18) Synovial Sarcoma
    Article Snippet: .. SYO-1 cells containing Tet-Off vector (Clontech) were isolated with G418 sulfate (1 mg/ml). ..

    Transfection:

    Article Title: Thioredoxin-interacting protein regulates the differentiation of murine erythroid precursors.
    Article Snippet: Thioredoxin-interacting protein regulates the differentiation of murine erythroid precursors Jadwiga J. Gasiorek, Marc Mikhael, Daniel Garcia-Santos, Simon T. Hui, Prem Ponka, and Volker Blank Lady Davis Institute for Medical Research, Montreal, Quebec, Canada; Department of Medicine, McGill University, Montreal, Quebec, Canada; Department of Physiology, McGill University, Montreal, Quebec, Canada; Division of Cardiology, Department of Medicine, University of California, Los Angeles, CA, USA

    Electroporation:

    Article Title: Thioredoxin-interacting protein regulates the differentiation of murine erythroid precursors.
    Article Snippet: Thioredoxin-interacting protein regulates the differentiation of murine erythroid precursors Jadwiga J. Gasiorek, Marc Mikhael, Daniel Garcia-Santos, Simon T. Hui, Prem Ponka, and Volker Blank Lady Davis Institute for Medical Research, Montreal, Quebec, Canada; Department of Medicine, McGill University, Montreal, Quebec, Canada; Department of Physiology, McGill University, Montreal, Quebec, Canada; Division of Cardiology, Department of Medicine, University of California, Los Angeles, CA, USA

    Selection:

    Article Title: Thioredoxin-interacting protein regulates the differentiation of murine erythroid precursors.
    Article Snippet: Thioredoxin-interacting protein regulates the differentiation of murine erythroid precursors Jadwiga J. Gasiorek, Marc Mikhael, Daniel Garcia-Santos, Simon T. Hui, Prem Ponka, and Volker Blank Lady Davis Institute for Medical Research, Montreal, Quebec, Canada; Department of Medicine, McGill University, Montreal, Quebec, Canada; Department of Physiology, McGill University, Montreal, Quebec, Canada; Division of Cardiology, Department of Medicine, University of California, Los Angeles, CA, USA

    Derivative Assay:

    Article Title: Calcium channel proteins
    Article Snippet: In the Clontech Tet OFF system the gene is not expressed (or expressed at very low levels) in the presence of doxycycline and expressed at very high levels in the absence of doxycycline. .. The pETE vector is derived from the commercially available Tet OFF vector (Clontech) by inserting an EBNA-1 element to confer an episomal property on the vector. ..

    Clone Assay:

    Article Title: Epidermal Growth Factor Receptor Variant III–Induced Glioma Invasion Is Mediated through Myristoylated Alanine-Rich Protein Kinase C Substrate Overexpression
    Article Snippet: .. EGFRvIII was cloned into the tetracycline-inducible Tet-Off vector (pTRE; Clontech) and cotransfected with the regulatory plasmid (pTA) into human U87 and U373 astrocytoma cells. .. Clones expressing the pTRE-EGFRvIII (U87vIII and U373vIII) or the empty pTRE vector (U87P and U373P) were grown in DMEM supplemented with 10% tetracycline-approved fetal bovine serum (Clontech).



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